Retinoblastoma is the most common intraocular tumor occurring in childhood. The incidence of this tumor is approximately 1/20,000 live births. The clinical diagnosis of this tumor has been facilitated by advances such as indirect ophthalmoscopy, ultrasonogrphy and an increased clinical awareness of the signs and symptoms of the tumor. Laboratory studies such as aqueous cytology and enzymology have also contributed to the diagnosis of this tumor. The objective of this research is to investigate the presence of retinoblastoma associated antigens from intraocular tumors, aqueous samples or spent tissue culture media. Antibodies prepared by standard protocols to purified retinoblastoma associated antigens will be utilized in the development of a radioimmunoassay for the examination of aqueous fluid from patients clinically suspected of harboring a retinoblastoma.